flow cytometry lights and lenses

Flow Cytometry Shared Resource

Overview

The Flow Cytometry Shared Resource provides Vanderbilt-Ingram Cancer Center investigators with advanced instrumentation and expert guidance for the analysis and isolation of individual cells. The resource supports studies requiring detailed characterization of complex cell populations through multiparameter flow cytometry, spectral cytometry, and high-speed cell sorting technologies. The Flow Cytometry Shared Resource collaborates with investigators to design experiments, optimize workflows, and generate high-quality data from a wide range of biological samples. By combining specialized expertise with state-of-the-art instrumentation, the resource provides a comprehensive platform for single-cell analysis and cell population characterization.


 

Flow Cytometry prisms

Directors

Services

Training and User Support
  • Instrument training and user certification for the following instrument platforms:
    • BD LSR Fortessa
    • BD FACS Aria III
    • Cytek Aurora
  • Custom Experimental Design and Optimization available upon request
  • FlowJo Training and Analysis service

Traditional Flow Cytometry and Cell Sorting
  • Self-service access to trained users on 3- and 5-laser BD LSR Fortessas and BD FACSAriaIII cell sorters
  • Multicolor flow cytometry analysis (up to 18 fluorochromes)
  • Bulk and Single-cell sorting
  • Multi-way sorting up to 4 populations simultaneously

Spectral Flow Cytometry and Cell Sorting
  • Self-service access to trained users on 4-Laser Cytek Aurora and 5-Laser Cytek Aurora CS cell sorters
  • Multicolor flow cytometry analysis with panels containing 40+ colors
  • Bulk and Single-cell sorting
  • Multi-way sorting up to 6 populations simultaneously

Experimental Design and Data Analysis
  • Experimental design consultation
  • Panel design consultation
  • Sample handling consultation
  • Staining protocol development and optimization
  • Data analysis software access and training

Contact Information

For more information about the Flow Cytometry Shared Resource services, please contact David Flaherty by email.

iLab: Flow Cytometry Shared Resource Core (FCSR)
Flow Cytometry Shared Resource