Genome Editing Shared Resource
Overview
The Genome Editing Shared Resource provides investigators at the Vanderbilt-Ingram Cancer with expertise and technologies for creating genetically engineered mouse models and human stem cell models that advance cancer research. The resource offers end-to-end support for genome editing projects, including model design, validation, generation, preservation, and distribution. The Genome Editing Shared Resource also maintains specialized capabilities for cryopreservation, repository management, and model recovery, ensuring valuable research resources remain accessible to the scientific community. By bringing together model generation and repository services, this resource provides investigators with reliable access to customized and well-characterized research models.
Directors
Mark Magnuson, MD
Scientific Director
Jennifer Skelton, MS
Managing Director
Services
Genome Editing & Model Generation
- CRISPR/Cas9 genome editing design and implementation in mouse embryos and human pluripotent stem cells
- Genetically engineered mouse model generation
- Knockout, knock-in, conditional, and humanized model development
- Transgenic and gene-targeted animal model development
- Mouse zygote microinjection and embryo manipulation services
- Founder screening, breeding strategy development, and line establishment
Stem Cell Engineering
- Human pluripotent stem cell (hPSC) genome engineering
- Clonal cell line isolation and characterization
- Validation of targeted genetic modifications
- Development of engineered stem cell models for disease research
Mouse Colony Preservation & Reproductive Services
- Embryo cryopreservation and recovery
- Sperm cryopreservation and quality assessment
- In vitro fertilization (IVF) and assisted reproductive technologies
- Mouse line rederivation, regeneration, and rescue
- Long-term mouse germplasm storage and repository services
- Importation, recovery, re-establishment of valuable mouse lines
Consultation & Research Support
- Genome editing project design and feasibility assessment
- Model generation strategy development
- Genotyping assay design and custom assay design for new strains with TransnetYX
- Cryopreservation and colony management consultation
- Guidance on model validation and quality control
- End-to-end support from project design through model generation, preservation, and distribution
Contact Information
For more information, contact Leesa Sampson or Jennifer Skelton.
For more information about the cryopreserved mouse repository, contact VCMR by email.